Simultaneous near-field and far-field fluorescence microscopy of single molecules
نویسندگان
چکیده
منابع مشابه
Simultaneous near-field and far-field fluorescence microscopy of single molecules.
A new microscope objective is presented for the parallel fluorescence detection below and above the critical angle of total internal reflection with single molecule sensitivity. The collection of supercritical angle fluorescence (SAF) leads to a strongly surface confined detection volume whereas the collection of undercritical angle fluorescence (UAF) allows for the observation of deeper axial ...
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ژورنال
عنوان ژورنال: Optics Express
سال: 2011
ISSN: 1094-4087
DOI: 10.1364/oe.19.006836